mkprobes
Start here
What SOLAR is and what these probes do
Before you start
Installation
Getting started
Troubleshooting
How-to, step by step
Build a dataset
Choose your targets
Design the codebook
Design probes
QC your panel
Order your oligos
Going further
SOLAR probesets for a new species
Assay context and under-the-hood logic
Dataflow map
Reference
CLI reference
Output columns, stage by stage
File formats and conventions
The SOLAR seqspec
Fidelity to the original implementation
Glossary
mkprobes
Index
Index
Symbols
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C
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D
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E
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G
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M
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O
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P
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S
Symbols
--add
mkprobes-suggest-targets command line option
--all
mkprobes-transcripts command line option
--allow
mkprobes-candidates command line option
--allow-file
mkprobes-run-panel command line option
--annotation
mkprobes-create-dataset command line option
--annotation-table
mkprobes-ingest command line option
--appris
mkprobes-transcripts command line option
--bcidx
mkprobes-draw-spec command line option
mkprobes-init command line option
mkprobes-validate-pool command line option
--blocklist-biotypes
mkprobes-ingest command line option
--blocklist-fasta
mkprobes-create-dataset command line option
--canonical
mkprobes-transcripts command line option
--codebook
mkprobes-construct command line option
mkprobes-validate-pool command line option
--dataset
mkprobes-init command line option
--debug
mkprobes command line option
--delete
mkprobes-assemble-MANIFEST-short command line option
--disallow
mkprobes-candidates command line option
--dpi
mkprobes-draw-spec command line option
--ensembl
mkprobes-transcripts command line option
--existing-codebook
mkprobes-make-codebook command line option
--expression
mkprobes-make-codebook command line option
--expression-column
mkprobes-make-codebook command line option
--extract
mkprobes-ingest command line option
--fasta
mkprobes-candidates command line option
mkprobes-create-dataset command line option
--fasta-key-regex
mkprobes-create-dataset command line option
mkprobes-ingest command line option
--force
mkprobes-init command line option
--fpkm-path
mkprobes-screen command line option
--fpkm_path
mkprobes-screen command line option
--gencode
mkprobes-transcripts command line option
--gene
mkprobes-candidates command line option
mkprobes-construct command line option
mkprobes-transcripts command line option
--gene-name-column
mkprobes-create-dataset command line option
mkprobes-ingest command line option
--genefile
mkprobes-transcripts command line option
--genes
mkprobes-filter-genes command line option
--genome
mkprobes-ingest command line option
--gtf
mkprobes-create-dataset command line option
mkprobes-ingest command line option
--have
mkprobes-suggest-targets command line option
--headerfooter
mkprobes-assemble command line option
--ignore-revcomp
mkprobes-candidates command line option
--iterations
mkprobes-make-codebook command line option
--keep-genome
mkprobes-ingest command line option
--layer
mkprobes-suggest-targets command line option
--limit
mkprobes-validate-pool command line option
--list-failed
mkprobes-run-panel command line option
--list-failed-all
mkprobes-run-panel command line option
--longest
mkprobes-transcripts command line option
--maxoverlap
mkprobes-run-panel command line option
mkprobes-screen command line option
--min-probes
mkprobes-filter-genes command line option
--minimum
mkprobes-run-panel command line option
mkprobes-screen command line option
--mode
mkprobes-convert-to-transcripts command line option
--n-bits
mkprobes-make-codebook command line option
--n-components
mkprobes-suggest-targets command line option
--no-strip-version
mkprobes-create-dataset command line option
mkprobes-ingest command line option
--offset
mkprobes-make-codebook command line option
--out
mkprobes-draw-spec command line option
mkprobes-filter-genes command line option
mkprobes-make-codebook command line option
mkprobes-suggest-targets command line option
--output
mkprobes-candidates command line option
mkprobes-run-panel command line option
--overlap
mkprobes-screen command line option
--overwrite
mkprobes-candidates command line option
mkprobes-create-dataset command line option
mkprobes-ingest command line option
mkprobes-run-panel command line option
mkprobes-screen command line option
--pseudogene-limit
mkprobes-candidates command line option
--restriction
mkprobes-construct command line option
mkprobes-run-panel command line option
mkprobes-screen command line option
--rm-species
mkprobes-assemble-MANIFEST-gen command line option
--rrna-fasta
mkprobes-ingest command line option
--score-pcs
mkprobes-suggest-targets command line option
--seed
mkprobes-make-codebook command line option
mkprobes-suggest-targets command line option
--skip-repeatmasker
mkprobes-assemble-MANIFEST-gen command line option
--spec
mkprobes-draw-spec command line option
mkprobes-validate-pool command line option
--species
mkprobes-create-dataset command line option
mkprobes-ingest command line option
mkprobes-init command line option
mkprobes-prepare command line option
--strip-version
mkprobes-create-dataset command line option
mkprobes-ingest command line option
--target-probes
mkprobes-construct command line option
mkprobes-run-panel command line option
--target_probes
mkprobes-construct command line option
--threads
mkprobes-prepare command line option
--trna-fasta
mkprobes-ingest command line option
--validate-only
mkprobes-ingest command line option
--verbose
mkprobes-assemble-MANIFEST-short command line option
mkprobes-transcripts command line option
--which
mkprobes-draw-spec command line option
--workers
mkprobes-run-panel command line option
-c
mkprobes-construct command line option
mkprobes-validate-pool command line option
-e
mkprobes-make-codebook command line option
-g
mkprobes-candidates command line option
mkprobes-construct command line option
mkprobes-filter-genes command line option
-j
mkprobes-run-panel command line option
-l
mkprobes-screen command line option
-m
mkprobes-convert-to-transcripts command line option
-N
mkprobes-construct command line option
-n
mkprobes-suggest-targets command line option
-o
mkprobes-candidates command line option
mkprobes-draw-spec command line option
mkprobes-filter-genes command line option
mkprobes-make-codebook command line option
mkprobes-run-panel command line option
mkprobes-suggest-targets command line option
-r
mkprobes-candidates command line option
-s
mkprobes-create-dataset command line option
mkprobes-ingest command line option
mkprobes-prepare command line option
-t
mkprobes-prepare command line option
-v
mkprobes-assemble-MANIFEST-short command line option
mkprobes-transcripts command line option
C
CODEBOOK
mkprobes-run-panel command line option
D
DATASET_DIR
mkprobes-ingest command line option
E
EXPRESSION
mkprobes-suggest-targets command line option
G
GENE
mkprobes-run-panel command line option
mkprobes-screen command line option
GENES
mkprobes-chkgenes command line option
mkprobes-convert-to-transcripts command line option
mkprobes-make-codebook command line option
M
MANIFEST
mkprobes-assemble command line option
mkprobes-check-manifest command line option
mkprobes command line option
--debug
mkprobes-assemble command line option
--headerfooter
MANIFEST
mkprobes-assemble-MANIFEST-gen command line option
--rm-species
--skip-repeatmasker
mkprobes-assemble-MANIFEST-short command line option
--delete
--verbose
-v
SHORT
mkprobes-candidates command line option
--allow
--disallow
--fasta
--gene
--ignore-revcomp
--output
--overwrite
--pseudogene-limit
-g
-o
-r
PATH
mkprobes-check-manifest command line option
MANIFEST
mkprobes-chkgenes command line option
GENES
PATH
mkprobes-construct command line option
--codebook
--gene
--restriction
--target-probes
--target_probes
-c
-g
-N
OUTPUT_PATH
PATH
mkprobes-convert-to-transcripts command line option
--mode
-m
GENES
PATH
mkprobes-create-dataset command line option
--annotation
--blocklist-fasta
--fasta
--fasta-key-regex
--gene-name-column
--gtf
--no-strip-version
--overwrite
--species
--strip-version
-s
PATH
mkprobes-draw-spec command line option
--bcidx
--dpi
--out
--spec
--which
-o
mkprobes-filter-genes command line option
--genes
--min-probes
--out
-g
-o
OUTPUT_PATH
mkprobes-hash command line option
PATH
mkprobes-ingest command line option
--annotation-table
--blocklist-biotypes
--extract
--fasta-key-regex
--gene-name-column
--genome
--gtf
--keep-genome
--no-strip-version
--overwrite
--rrna-fasta
--species
--strip-version
--trna-fasta
--validate-only
-s
DATASET_DIR
mkprobes-init command line option
--bcidx
--dataset
--force
--species
PROJECT
mkprobes-make-codebook command line option
--existing-codebook
--expression
--expression-column
--iterations
--n-bits
--offset
--out
--seed
-e
-o
GENES
PATH
mkprobes-prepare command line option
--species
--threads
-s
-t
PATH
mkprobes-provenance command line option
PATH
mkprobes-run-panel command line option
--allow-file
--list-failed
--list-failed-all
--maxoverlap
--minimum
--output
--overwrite
--restriction
--target-probes
--workers
-j
-o
CODEBOOK
GENE
PATH
mkprobes-screen command line option
--fpkm-path
--fpkm_path
--maxoverlap
--minimum
--overlap
--overwrite
--restriction
-l
GENE
OUTPUT_PATH
mkprobes-suggest-targets command line option
--add
--have
--layer
--n-components
--out
--score-pcs
--seed
-n
-o
EXPRESSION
mkprobes-transcripts command line option
--all
--appris
--canonical
--ensembl
--gencode
--gene
--genefile
--longest
--verbose
-v
PATH
mkprobes-validate-pool command line option
--bcidx
--codebook
--limit
--spec
-c
POOL
O
OUTPUT_PATH
mkprobes-construct command line option
mkprobes-filter-genes command line option
mkprobes-screen command line option
P
PATH
mkprobes-candidates command line option
mkprobes-chkgenes command line option
mkprobes-construct command line option
mkprobes-convert-to-transcripts command line option
mkprobes-create-dataset command line option
mkprobes-hash command line option
mkprobes-make-codebook command line option
mkprobes-prepare command line option
mkprobes-provenance command line option
mkprobes-run-panel command line option
mkprobes-transcripts command line option
POOL
mkprobes-validate-pool command line option
PROJECT
mkprobes-init command line option
S
SHORT
mkprobes-assemble-MANIFEST-short command line option